Several questions surround DNA test kits. For the consumer, the process is simple and takes little time. However, after the sample returns to the DNA lab, things are a bit more complicated. Each vial, from each DNA test kit, must be extracted carefully so as to not damage the delicate strands. So what steps go into the DNA extraction process? In today’s blog, Dynamic DNA Labs describes the extraction process and how your DNA goes from the kit to becoming accurate information.
Step 1: Lysis
Upon return to the DNA lab, the vial from the test needs to be carefully examined to make sure the consumer completed it correctly. The extraction process starts with lysis, the term for breaking open the cells to access the nucleus. Breaking open each cell allows you to access the strands, which are held within the core.
Step 2: Separation
After breaking the cell open, there will be cellular debris floating throughout the key extraction areas. A separation process takes place to get the DNA as clean as possible. There are many ways to clean theses strands; however, a common practice is adding a protein enzyme to break out this additional debris. Ultimately, this will isolate any strand from the remainder of the cell for simple extraction.
Step 3: Precipitation
The separation step isolates DNA strands for sampling, ensuring careful handling. You must precipitate this DNA sample with alcohol, often isopropanol. Make sure this is 100% alcohol or else it can destroy the sample entirely. After adding the alcohol, you will begin to see white strands that look stringy. These will be spooled out and properly cleaned again.
Step 4: Cleansing
This final handling stage of the extraction process is utilized to get the purest form of DNA possible. Upon cleaning, the DNA is re-dissolved into pure water for storage.
Step 5: Post-Examination
Finally, re-evaluate your extraction process thoroughly. Within the DNA lab, clean all equipment used to avoid cross-contamination between DNA samples. The sample should be stored in a temperature controlled room, with little contact to heat. For long term preservation, DNA can be stored in temperatures ranging from -20°C to -196°C.
Participate in DNA Testing with Dynamic DNA Labs Today
Dynamic DNA Labs is a highly qualified business that specializes in DNA testing. Within our state-of-the-art DNA labs, we can dissect DNA test kits quickly while maintaining quality. Your DNA tells you many details you may not have known about yourself, and can even tell you more about your nutrition, fitness, and especially genealogy. The extraction process is the most critical step in delivering you accurate and reliable information.